CFX96TOUCH Fluorescent Quantitative PCR ntsuas

CFX96Touch fluorescent quantitative PCR tuaj yeem siv rau hauv ntau yam kev tshawb fawb ntawm nucleic acid kom muaj nuj nqis, gene qhia theem tsom xam, kuaj noob hloov pauv, kuaj pom GMO, thiab kev tshuaj xyuas cov khoom tshwj xeeb.

Ua haujlwm ib puag ncig:
1.1 Ua haujlwm kub: 5-31 ° C
1.2 Ua haujlwm thiab av noo: txheeb ze av noo ≤80%
1.3 Ua haujlwm fais fab: 100-240 VAC, 50-60Hz
Kev ua tau zoo thiab kev xav tau ntawm CFX96Touch fluorescence quantitative PCR
3.1 Kev ua haujlwm tseem ceeb (* yog qhov ntsuas uas yuav tsum tau ua)
* 3.1.1 Rau qhov kev sim ntsuas, 5% PCR tuaj yeem pom tau, thiab 5 lub hom phiaj tuaj yeem kuaj pom tib lub sijhawm, thiab qhov tshwj xeeb FRET nrhiav kom pom channel yog ib txhij kuaj.
* 3.1.2 Nrog rau qhov kub thiab txias gradient PCR muaj nuj nqi, koj tuaj yeem khiav 8 qhov kub sib txawv tib lub sijhawm, txhua qhov kub kub incubation.
3.1.3 Qhib cov reagents tiav, ntau yam kev tshawb fawb thiab kev kho cov tshuaj reagents;
3.1.4 Haum rau ntau yam fluorescence txoj kev xws li Taqman, Molecular Beacon, Fret sojntsuam, Sybr Green i, thiab lwm yam .;
3.1.5 Qhib, 0.2ml ib leeg raj, octal, 96-zoo phaj, thiab lwm yam.
* 3.1.6 tuaj yeem khiav ntawm nws tus kheej, ua haujlwm offline tiag tiag, tsis tas yuav txuas lub computer los saib xyuas PCR fluorescent amplification nkhaus hauv lub sijhawm;
3.2 Cov kev cai tseem ceeb (* yog qhov taw qhia uas yuav tsum tau ua)
* 3.2.1 Qauv muaj peev xwm: 96 × 0.2ml, txheem specifications 96-zoo daim hlau (12 × 8) yuav siv tau;
3.2.2 Khoom siv: 0.2ml ib lub raj, yim interlocking, 96-zoo daim hlau, thiab lwm yam.
3.2.3 Reaction System: 1-50μL (pom zoo 10-25 μL);
* 3.2.4 Lub teeb ci: Rau LEDs nrog cov ntxaij lim dej;
* 3.2.5 Ntes: Rau photosensitive diodes nrog cov ntxaij lim dej;
* 3.2.6 liter txias ceev: 5 ° C / sec;
3.2.7 Kev tswj qhov kub thiab txias: 0 -100 ° C;
3.2.8 Kub qhov tseeb: ± 0.2 ° C (90 ˚C);
3.2.9 Temperature uniformity: ± 0.4 ° C (90 ˚C hauv 10 vib nas this);
* 3.2.10 Dynamic Temperature Gradient Function: Khiav 8 qhov kub sib txawv tib lub sijhawm; gradient kub tswj ntau yam: 30 -100 ° C; gradient kub sib txawv ntau yam: 1 - 24 ° C; gradient kub incubation lub sij hawm: tib yam;
3.2.11 excitation / emission wavelength ntau: 450-730 nm;
3.2.12 rhiab heev: Ib daim qauv ntawm tib neeg genome tuaj yeem kuaj pom;
3.2.13 Dynamic ntau yam: 10 qhov ntau;
3.2.14 Zaub: 8.5 nti xim kov npo;
3.2.15 Cov Ntaub Ntawv Kev Ntsuam Xyuas Hom: Tus Qauv Curve Quantity, Melting Curve, CT lossis ΔΔCT Gene Expression Analysis, Multiple Internal Genodes Analysis thiab Amplification Efficiency Calculation, Multiple Data Files Gene Expression Analysis, Allelic Analysis, Type Analysis, have Gene, melting curve analysis function ;
3.2.16 Cov Ntaub Ntawv Export: Excel, Lo Lus, lossis PowerPoint. Cov neeg siv daim ntawv tshaj tawm muaj cov chaw khiav, cov duab kos, thiab cov ntaub ntawv cov ntaub ntawv, uas tuaj yeem luam tawm lossis khaws cia ua PDF;
* 3.2.17 Chromosomal qauv kev tshawb fawb: Ib txoj hauv kev ntawm kev soj ntsuam ntau ntawm cov qauv chromatin los ntawm kev sib piv lub luag haujlwm ntawm genomic DNA degradation los ntawm kev sib piv lub luag haujlwm ntawm genomic DNA. Nws yeej ua pov thawj qhov siab correlation ntawm tus qauv chromatin thiab gene qhia;